Journal article
Pair correlation microscopy reveals the role of nanoparticle shape in intracellular transport and site of drug release
E Hinde, K Thammasiraphop, HTT Duong, J Yeow, B Karagoz, C Boyer, JJ Gooding, K Gaus
Nature Nanotechnology | NATURE PUBLISHING GROUP | Published : 2017
Abstract
Nanoparticle size, surface charge and material composition are known to affect the uptake of nanoparticles by cells. However, whether nanoparticle shape affects transport across various barriers inside the cell remains unclear. Here we used pair correlation microscopy to show that polymeric nanoparticles with different shapes but identical surface chemistries moved across the various cellular barriers at different rates, ultimately defining the site of drug release. We measured how micelles, vesicles, rods and worms entered the cell and whether they escaped from the endosomal system and had access to the nucleus via the nuclear pore complex. Rods and worms, but not micelles and vesicles, ent..
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Grants
Awarded by Türkiye Bilimsel ve Teknolojik Araştirma Kurumu
Funding Acknowledgements
E.H. is funded by a Cancer Institute NSW Early Career Fellowship (RG151879) and UNSW Vice Chancellor Research Fellowship. B.K. acknowledges the Scientific and Technological Research Council of Turkey (TUBITAK) for financial support. C.B. is funded by a Future Fellowship (FT1210096) from Australian Research Council (ARC). K.G. acknowledges funding from the ARC Centre of Excellence in Advanced Molecular Imaging (CE140100011) and National Health and Medical Research Council of Australia (1059278, 1037320). J.J.G. acknowledges funding from the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology (CE140100036), the ARC Laureate Fellowship (FL150100060) program and a National Health and Medical Research Council program grant (1091261). The authors thank C. Benzing (UNSW) for discussion on cell uptake and endosomal escape. The authors thank E. Gratton (University of California, Irvine) for discussion on data analysis. The work was supported by the BioMedical Imaging Facility at UNSW.